abstract：:Cell types more advanced in development than embryonic stem cells, such as EpiSCs, fail to contribute to chimeras when injected into pre-implantation-stage blastocysts, apparently because the injected cells undergo apoptosis. Here we show that transient promotion of cell survival through expression of the anti-apoptot...

journal_title：Cell stem cell

authors： Masaki H,Kato-Itoh M,Takahashi Y,Umino A,Sato H,Ito K,Yanagida A,Nishimura T,Yamaguchi T,Hirabayashi M,Era T,Loh KM,Wu SM,Weissman IL,Nakauchi H

更新日期：2016-11-03 00:00:00

abstract：:Mammalian skin is a complex and heterogeneous tissue with several distinct compartments and stem cell populations. Joost et al. (2016) now use single-cell RNaseq to comprehensively reconstruct this complexity, revealing spatial and pseudotemporal differences between transcriptional programs in distinct compartments an...

journal_title：Cell stem cell

authors： Chen JC,Christiano AM

更新日期：2016-10-06 00:00:00

abstract：:Like politics, most developmental signals are local. However, in this issue of Cell Stem Cell, Kwan et al. (2016) and colleagues describe how a stress-induced signal that originates in the zebrafish brain promotes the formation of blood at a distant site, the dorsal aorta. ...

journal_title：Cell stem cell

authors： Speck NA

更新日期：2016-09-01 00:00:00

abstract：:Hematopoiesis is a complex process that requires a high degree of transcriptional diversification during lineage commitment and differentiation. de Graaf et al. (2016) have now generated a comprehensive gene expression dataset that allows cell-type-specific genes as well as associated transcription factor expression p...

journal_title：Cell stem cell

authors： Drissen R,Nerlov C

更新日期：2016-08-04 00:00:00

abstract：:Neurons derived from human pluripotent stem cells (hPSCs) are powerful tools for studying human neural development and diseases. Robust functional coupling of hPSC-derived neurons with target tissues in vitro is essential for modeling intercellular physiology in a dish and to further translational studies, but it has ...

journal_title：Cell stem cell

authors： Oh Y,Cho GS,Li Z,Hong I,Zhu R,Kim MJ,Kim YJ,Tampakakis E,Tung L,Huganir R,Dong X,Kwon C,Lee G

更新日期：2016-07-07 00:00:00

abstract：:Leukemia stem cells (LSCs) are resistant to current therapies used to treat chronic myeloid leukemia (CML). Abraham et al. (2016) have identified a molecular network critical for CML LSC survival and propose that simultaneously targeting two of their major transcriptional regulators, p53 and c-Myc, may facilitate thei...

journal_title：Cell stem cell

authors： Carvajal LA,Steidl U

更新日期：2016-07-07 00:00:00

abstract：:Cell engineering has brought us tantalizingly close to the goal of deriving patient-specific hematopoietic stem cells (HSCs). While directed differentiation and transcription factor-mediated conversion strategies have generated progenitor cells with multilineage potential, to date, therapy-grade engineered HSCs remain...

journal_title：Cell stem cell

authors： Rowe RG,Mandelbaum J,Zon LI,Daley GQ

更新日期：2016-06-02 00:00:00

abstract：:The suspected link between infection by Zika virus (ZIKV), a re-emerging flavivirus, and microcephaly is an urgent global health concern. The direct target cells of ZIKV in the developing human fetus are not clear. Here we show that a strain of the ZIKV, MR766, serially passaged in monkey and mosquito cells efficientl...

journal_title：Cell stem cell

authors： Tang H,Hammack C,Ogden SC,Wen Z,Qian X,Li Y,Yao B,Shin J,Zhang F,Lee EM,Christian KM,Didier RA,Jin P,Song H,Ming GL

更新日期：2016-05-05 00:00:00

abstract：:Fanconi anemia (FA) is an inherited DNA repair disorder characterized by progressive bone marrow failure (BMF) from hematopoietic stem and progenitor cell (HSPC) attrition. A greater understanding of the pathogenesis of BMF could improve the therapeutic options for FA patients. Using a genome-wide shRNA screen in huma...

journal_title：Cell stem cell

authors： Zhang H,Kozono DE,O'Connor KW,Vidal-Cardenas S,Rousseau A,Hamilton A,Moreau L,Gaudiano EF,Greenberger J,Bagby G,Soulier J,Grompe M,Parmar K,D'Andrea AD

更新日期：2016-05-05 00:00:00

abstract：:An improved understanding of the bone marrow failure (BMF) mechanisms in Fanconi anemia (FA) may improve current therapeutic strategies. Zhang et al. identify hyperactive TGF-β signaling as an underlying cause of BMF in FA mice and patient cells, whose inhibition promotes DNA repair and hematopoietic stem and progenit...

journal_title：Cell stem cell

authors： Tummala H,Dokal I

更新日期：2016-05-05 00:00:00

abstract：:Substantial expansion in the number of cerebral cortex neurons is thought to underlie cognitive differences between humans and other primates, although the mechanisms underlying this expansion are unclear. Otani et al. (2016) utilize PSC-derived brain organoids to study how species-specific differences in cortical pro...

journal_title：Cell stem cell

authors： Dyer MA

更新日期：2016-04-07 00:00:00

abstract：:Developing technologies for efficient and scalable disruption of gene expression will provide powerful tools for studying gene function, developmental pathways, and disease mechanisms. Here, we develop clustered regularly interspaced short palindromic repeat interference (CRISPRi) to repress gene expression in human i...

journal_title：Cell stem cell

authors： Mandegar MA,Huebsch N,Frolov EB,Shin E,Truong A,Olvera MP,Chan AH,Miyaoka Y,Holmes K,Spencer CI,Judge LM,Gordon DE,Eskildsen TV,Villalta JE,Horlbeck MA,Gilbert LA,Krogan NJ,Sheikh SP,Weissman JS,Qi LS,So PL,Conk

更新日期：2016-04-07 00:00:00

abstract：:We are only beginning to understand the higher-order chromatin structure of pluripotent stem cells and its relevance to cell fate. Three recent studies used different approaches to reconstruct the 3D chromatin landscape of naive and primed pluripotent cells, unveiling common features as well as differences between the...

journal_title：Cell stem cell

authors： Wei Z,Lu W

更新日期：2016-02-04 00:00:00

abstract：:Pluripotent stem cells are defined by their capacity to differentiate into all three tissue layers that comprise the body. Chimera formation, generated by stem cell transplantation to the embryo, is a stringent assessment of stem cell pluripotency. However, the ability of human pluripotent stem cells (hPSCs) to form e...

journal_title：Cell stem cell

authors： Mascetti VL,Pedersen RA

更新日期：2016-01-07 00:00:00

abstract：:The identification of mechanisms that regulate stem cell renewal has led to the development of innovative approaches to expand hematopoietic stem cells ex vivo. One approach reported by Wagner et al. (2016) in this issue of Cell Stem Cell has demonstrated dramatic expansion of umbilical cord blood stem cells that prom...

journal_title：Cell stem cell

authors： Nikiforow S,Ritz J

更新日期：2016-01-07 00:00:00

abstract：:We have recently demonstrated that reactive glial cells can be directly reprogrammed into functional neurons by a single neural transcription factor, NeuroD1. Here we report that a combination of small molecules can also reprogram human astrocytes in culture into fully functional neurons. We demonstrate that sequentia...

journal_title：Cell stem cell

authors： Zhang L,Yin JC,Yeh H,Ma NX,Lee G,Chen XA,Wang Y,Lin L,Chen L,Jin P,Wu GY,Chen G

更新日期：2015-12-03 00:00:00

abstract：:The identification of tumor-initiating cells represents a significant challenge for studies of prostate cancer. In a recent issue of Cell Reports, Agarwal and colleagues use organoid culture to ascertain two distinct luminal progenitors in a mouse model of prostate cancer, shedding new light on lineage relationships i...

journal_title：Cell stem cell

authors： Shen MM

更新日期：2015-12-03 00:00:00

abstract：:Recurrent mutations in cohesin complex proteins have been identified in pre-leukemic hematopoietic stem cells and during the early development of acute myeloid leukemia and other myeloid malignancies. Although cohesins are involved in chromosome separation and DNA damage repair, cohesin complex functions during hemato...

journal_title：Cell stem cell

authors： Mazumdar C,Shen Y,Xavy S,Zhao F,Reinisch A,Li R,Corces MR,Flynn RA,Buenrostro JD,Chan SM,Thomas D,Koenig JL,Hong WJ,Chang HY,Majeti R

更新日期：2015-12-03 00:00:00

abstract：:Trophoblast stem cells (TSCs) arise from the first cell fate decision in the developing embryo and generate extra-embryonic lineages, giving rise to the fetal portion of the placenta. Mouse embryonic and extra-embryonic lineages are strictly separated by a distinct epigenetic barrier, which is not fully overcome follo...

journal_title：Cell stem cell

authors： Kubaczka C,Senner CE,Cierlitza M,Araúzo-Bravo MJ,Kuckenberg P,Peitz M,Hemberger M,Schorle H

更新日期：2015-11-05 00:00:00

abstract：:In this issue of Cell Stem Cell, Saito et al. (2015) examine how leukemia-initiating cells react to metabolic stress imposed by different tissue environments. They find that inhibition of the metabolic stress sensor AMP-activated protein kinase (AMPK) led to deranged glucose metabolism and redox homeostasis, resulting...

journal_title：Cell stem cell

authors： Shaw RJ

更新日期：2015-11-05 00:00:00

abstract：:Patients with type 1 diabetes (T1D) often experience gastrointestinal enteropathy (DE) of unclear etiology. Now in Cell Stem Cell, D'Addio et al. (2015) utilize organoid culture models to study the roles of stem cells in DE and show that circulating IGF/IGFBP3 controls colonic stem cell function during homeostasis and...

journal_title：Cell stem cell

authors： Cheng CW,Yilmaz ÖH

更新日期：2015-10-01 00:00:00

abstract：:Mouse embryonic stem cells (mESCs) cultured under serum/LIF conditions exhibit heterogeneous expression of pluripotency-associated factors that can be overcome by two inhibitors (2i) of the MEK and GSK3 pathways. Several studies have shown that the "ground state" induced by 2i is characterized by global hypomethylatio...

journal_title：Cell stem cell

authors： Galonska C,Ziller MJ,Karnik R,Meissner A

更新日期：2015-10-01 00:00:00

abstract：:Cardiac stem/progenitor cells hold great potential for regenerative therapies; however, the mechanisms regulating their expansion and differentiation remain insufficiently defined. Here we show that Ldb1 is a central regulator of genome organization in cardiac progenitor cells, which is crucial for cardiac lineage dif...

journal_title：Cell stem cell

authors： Caputo L,Witzel HR,Kolovos P,Cheedipudi S,Looso M,Mylona A,van IJcken WF,Laugwitz KL,Evans SM,Braun T,Soler E,Grosveld F,Dobreva G

更新日期：2015-09-03 00:00:00

abstract：:Acute Myelogenous Leukemia (AML) is an aggressive cancer that strikes both adults and children and is frequently resistant to therapy. Thus, identifying signals needed for AML propagation is a critical step toward developing new approaches for treating this disease. Here, we show that Tetraspanin 3 is a target of the ...

journal_title：Cell stem cell

authors： Kwon HY,Bajaj J,Ito T,Blevins A,Konuma T,Weeks J,Lytle NK,Koechlein CS,Rizzieri D,Chuah C,Oehler VG,Sasik R,Hardiman G,Reya T

更新日期：2015-08-06 00:00:00

abstract：:Regeneration of skin and hair follicles after wounding--a process known as wound-induced hair neogenesis (WIHN)--is a rare example of adult organogenesis in mammals. As such, WIHN provides a unique model system for deciphering mechanisms underlying mammalian regeneration. Here, we show that dsRNA, which is released fr...

journal_title：Cell stem cell

authors： Nelson AM,Reddy SK,Ratliff TS,Hossain MZ,Katseff AS,Zhu AS,Chang E,Resnik SR,Page C,Kim D,Whittam AJ,Miller LS,Garza LA

更新日期：2015-08-06 00:00:00

abstract：:Understanding the mechanisms underlying neuronal fate determination will provide important insights into brain development and regenerative approaches to neurological diseases. Now in Cell Stem Cell, Masserdotti et al. (2015) use neuronal conversion of astrocytes to dissect transcriptional mechanisms of fate determina...

journal_title：Cell stem cell

authors： Wang C,Fong H,Huang Y

更新日期：2015-07-02 00:00:00

abstract：:Aging is characterized by a decrease in genome integrity, impaired organ maintenance, and an increased risk of cancer, which coincide with clonal dominance of expanded mutant stem and progenitor cell populations in aging tissues, such as the intestinal epithelium, the hematopoietic system, and the male germline. Here ...

journal_title：Cell stem cell

authors： Adams PD,Jasper H,Rudolph KL

更新日期：2015-06-04 00:00:00

abstract：:Heterogeneity within the self-renewal durability of adult hematopoietic stem cells (HSCs) challenges our understanding of the molecular framework underlying HSC function. Gene expression studies have been hampered by the presence of multiple HSC subtypes and contaminating non-HSCs in bulk HSC populations. To gain deep...

journal_title：Cell stem cell

authors： Wilson NK,Kent DG,Buettner F,Shehata M,Macaulay IC,Calero-Nieto FJ,Sánchez Castillo M,Oedekoven CA,Diamanti E,Schulte R,Ponting CP,Voet T,Caldas C,Stingl J,Green AR,Theis FJ,Göttgens B

更新日期：2015-06-04 00:00:00

abstract：:Liao et al. (2015) recently reported on the effects of disrupting DNA methyltransferase activity in human embryonic stem cells (hESCs). This work highlights key differences between mammalian ESC models upon the loss of these essential proteins and provides comprehensive base resolution methylome maps of DNMT targets d...

journal_title：Cell stem cell

authors： Bogdanović O,Lister R

更新日期：2015-05-07 00:00:00

abstract：:While studies of organ development have traditionally relied on model organisms, recent advances in embryonic stem cell (ESC) culture allow investigation of organogenesis in human cells. Wang et al. (2015) employ this system to map the dynamic enhancer landscape during ESC differentiation to the endoderm derivatives p...

journal_title：Cell stem cell

authors： Kaestner KH

更新日期：2015-04-02 00:00:00

abstract：:Regulated blood production is achieved through the hierarchical organization of dormant hematopoietic stem cell (HSC) subsets that differ in self-renewal potential and division frequency, with long-term (LT)-HSCs dividing the least. The molecular mechanisms underlying this variability in HSC division kinetics are unkn...

journal_title：Cell stem cell

authors： Laurenti E,Frelin C,Xie S,Ferrari R,Dunant CF,Zandi S,Neumann A,Plumb I,Doulatov S,Chen J,April C,Fan JB,Iscove N,Dick JE

更新日期：2015-03-05 00:00:00

abstract：:Cancer stem cells (CSCs) are tumor cells that have the principal properties of self-renewal, clonal tumor initiation capacity, and clonal long-term repopulation potential. CSCs reside in niches, which are anatomically distinct regions within the tumor microenvironment. These niches maintain the principle properties of...

journal_title：Cell stem cell

authors： Plaks V,Kong N,Werb Z

更新日期：2015-03-05 00:00:00

abstract：:About half of the human genome consists of highly repetitive elements, most of which are considered dispensable for human life. Here, we report that repetitive elements originating from endogenous retroviruses (ERVs) are systematically transcribed during human early embryogenesis in a stage-specific manner. Our analys...

journal_title：Cell stem cell

authors： Göke J,Lu X,Chan YS,Ng HH,Ly LH,Sachs F,Szczerbinska I

更新日期：2015-02-05 00:00:00

abstract：:The Wnt signaling pathway controls stem cell identity in the intestinal epithelium and in many other adult organs. The transcription factor Ascl2 (a Wnt target gene) is a master regulator of intestinal stem cell identity. It is unclear how the continuous Wnt gradient along the crypt axis is translated into discrete ex...

journal_title：Cell stem cell

authors： Schuijers J,Junker JP,Mokry M,Hatzis P,Koo BK,Sasselli V,van der Flier LG,Cuppen E,van Oudenaarden A,Clevers H

更新日期：2015-02-05 00:00:00

abstract：:Twist1 promotes epithelial-to-mesenchymal transition (EMT), invasion, metastasis, and cancer stem cell (CSC) properties. However, it remains unclear whether Twist1 is also required for tumor initiation and whether Twist1-induced cancer stemness and EMT are functionally linked. Using a conditional deletion of Twist1 at...

journal_title：Cell stem cell

authors： Beck B,Lapouge G,Rorive S,Drogat B,Desaedelaere K,Delafaille S,Dubois C,Salmon I,Willekens K,Marine JC,Blanpain C

更新日期：2015-01-08 00:00:00

abstract：:Human induced pluripotent stem cells (hiPSCs) hold promise for myocardial repair following injury, but preclinical studies in large animal models are required to determine optimal cell preparation and delivery strategies to maximize functional benefits and to evaluate safety. Here, we utilized a porcine model of acute...

journal_title：Cell stem cell

authors： Ye L,Chang YH,Xiong Q,Zhang P,Zhang L,Somasundaram P,Lepley M,Swingen C,Su L,Wendel JS,Guo J,Jang A,Rosenbush D,Greder L,Dutton JR,Zhang J,Kamp TJ,Kaufman DS,Ge Y,Zhang J

更新日期：2014-12-04 00:00:00

abstract：:Modifications of mRNAs can have a profound effect on cellular function and differentiation. In this issue of Cell Stem Cell, Batista et al. (2014) describe fundamental parameters of N(6)-methyl-adenosine modification of mRNAs in embryonic stem cells and provide strong evidence that modification plays a role in exit fr...

journal_title：Cell stem cell

authors： Jalkanen AL,Wilusz J

更新日期：2014-12-04 00:00:00

abstract：:Two groups recently reported the in vitro differentiation of human embryonic stem cells into insulin-secreting cells, achieving an elusive goal for regenerative medicine. Herein we provide a perspective regarding these developments, compare phenotypes of the insulin-containing cells to human β cells, and discuss impli...

journal_title：Cell stem cell

authors： Kushner JA,MacDonald PE,Atkinson MA

更新日期：2014-11-06 00:00:00

abstract：:With the exception of imprinted genes and certain repeats, DNA methylation is globally erased during preimplantation development. Recent studies have suggested that Tet3-mediated oxidation of 5-methylcytosine (5mC) and DNA replication-dependent dilution both contribute to global paternal DNA demethylation, but demethy...

journal_title：Cell stem cell

authors： Shen L,Inoue A,He J,Liu Y,Lu F,Zhang Y

更新日期：2014-10-02 00:00:00

abstract：:Emerging evidence strongly suggests that stem cells and their differentiated progeny display distinct metabolic profiles, but how metabolic changes are coupled with organogenesis has remained unclear. Homem et al. (2014) now reveal a hormone-dependent pathway that couples metabolic changes with stem cell differentiati...

journal_title：Cell stem cell

authors： Buttitta L,Lee CY

更新日期：2014-09-04 00:00:00